The potential of aerosol eDNA sampling for the characterisation of commercial seed lots
Fig 5
Species-specific minibarcode frequencies from aerosol samples collected above a titration of maize + cowpea seed mixtures.
Frequency of maize and cowpea-specific mini-barcode sequences recovered from aerosol samples collected above individual grain bins in a titration series containing 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% (Coriolis samples) and 0%, 5%, 10%, 30%, 50%, 70%, 90%, 95% and 100% (vacuum samples) of maize grains in cowpea grains. (A) Histogram of the relative percentage of maize and cowpea mini-barcode sequences recovered from aerosol samples collected using a Coriolis aerosol sampler 60cm above bins representing the titration series of grain mixes. (B) Histogram of the relative percentage of maize and cowpea mini-barcode sequences recovered from aerosol samples collected using a hand-held, high power vacuum sampler 10cm above bins representing the titration series of grain mixes.