Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells
Fig 5
Flow cytometric analysis of CXCR4 protein expression in DE differentiated SNL-201B7 and -253G1 or MEFP1-201B7 and -253G1 cells.
The percentages of CXCR4-positive cells in DE differentiated SNL-201B7 (A) and -253G1 (B) or MEFP1-201B7 (A) and -253G1 cells (B) (n = 12 each) were analyzed by flow cytometry. Mouse IgG2a antibody was used as an isotype control. Dead cells were excluded by using 7-AAD. Data represent the mean ± SEM and are representative of more than two or three experiments. ***p < 0.001 versus SNL-201B7 and -253G1 cells.