The anticancer peptide RT53 induces immunogenic cell death
Fig 5
Intratumoral administration of RT53 induces tumor necrosis, immune cells infiltration and inflammation.
(A) Plasma was harvested 4, 24, 48 or 96 h following single intratumoral injection of normal saline (control) or 300 μg RT53 in normal saline and IL-1β (left panel) as well as IL-6 (right panel) levels were estimated using ELISA. Data from 3 animals are presented for each time point as mean±SEM. (B) Established MCA205 fibrosarcomas were surgically excised 24 h or 96 h post intratumoral injection with normal saline (control) or 300 μg RT53 in normal saline and sections subjected to H&E staining (top) or stained for CD3 or rabbit IgG isotype control (middle and bottom, respectively). (C) Number of infiltrating CD3-positive cells per view field following CD3 staining. Data from 3 animals are presented as mean±SEM. (D) Relative transcription of CCL2 and CXCL10 (normalized to GAPDH) as determined by real-time RT-PCR on samples of tumors 24 h after RT53 or normal saline injection. Values of CCL2 and CXCL10 are represented as fold change relative to untreated tumors, set to 1 (mean±SEM; n = 3).