Expression and purification of a functional heteromeric GABAA receptor for structural studies
Fig 2
Expression of GABAA receptors in oocytes.
(A) The α1/β2 receptor demonstrates increased expression levels and homogeneity relative to the α1 subunit alone. The arrow indicates a population reduction of smaller oligomers in the presence of the β2 subunit. (B) Co-injection of γ2S mRNA with the α1 subunit does not improve receptor homogeneity. (C) Injection of all three subunits results in attenuated expression levels relative to α1/β2. All FSEC traces were acquired from the same batch of oocytes. Absolute fluorescence intensities are plotted on the same scale.