The role of AKT and FOXO3 in preventing ovarian toxicity induced by cyclophosphamide
Fig 5
HPE inhibited protein expression of p-Rictor, bad, bax, PPAR and promoted protein expression of p-AKT and p-Foxo3a in POF model mice.
A. Ovaries were harvested at day 1 after the final administration of HPE or saline, equivalent amount of whole ovary detergent lysates was western blotted with indicated antibodies. Per lane represents individual animals (5–8 animals per group). B. Compared with control group, the level of p-AKT and p-Foxo3a in POF group was significantly lower, the level of p-Rictor, Bad, Bax, PPAR in POF group was significantly higher, (*P<0.05). Compared with POF group, the level of p-Rictor, Bad, Bax, PPAR, p-AKT and p-Foxo3a in CH+, CH ++ and CH +++ groups was significantly higher (*P<0.05). Data were pooled and represented as mean ± SD, n = 5–8 animals per group.