Antidepressive and anxiolytic effects of ostruthin, a TREK-1 channel activator
Fig 5
Antidepressive effect of ostruthin.
(A) Effect of ostruthin on the depression-like behavior of mice was examined using the forced-swim test. The amount of time spent immobile in the last 4 min of the swimming period (6 min) was recorded. Administration of ostruthin 30 min before test significantly reduced the time spent immobile in a dose-dependent manner. (B) Antidepressive effect of ostruthin in the tail-suspension test. The amount of time spent immobile was recorded for 5 min. (C) Insignificant effect on social defeat stress test. After the exposure to aggressor ICR mice for 9 days, C57BL/6J test mouse was placed in an open-field arena. Firstly the time spent around the empty enclosure and subsequently that with an unfamiliar ICR mouse was measured. The former duration was subtracted from the later. (D) Lack of effect on voluntary motility. After the end of the elevated plus maze, mice were subjected to voluntary wheel running test for 5 min. All experiments were carried out 30 min after the administration of PBS (0) or the indicated dose of ostruthin.