Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus
Fig 3
HPAEC chromatogram of endoxylanase pre-digested WAX incubated with concentrated compost extract.
PII extract (A) and extract of compost colonized by A15 (B), HGH43-1 (C), and HGH43-2 (D). Endoxylanase pre-digested WAX without compost extracts (1), with concentrated compost extract (2), and compost extract without WAX-oligomers added (3) are shown in each panel. The line closest to the x-axis shows the retention time of arabinose and xylose at 3.5 and 4.5 min, respectively. Each sample is representative for a biological triplicate. The chromatogram is magnified from retention time 12–16.5 min (A’, B’, C’, and D’) for each sample where single and double substituted arabinoxylan oligomers are indicated with S and D, respectively. Specific xylosyl oligomers substituted with a single arabinosyl are indicated with 4.1, 3.2 in A and A’. Xylosyl oligomers with a double arabinosyl substitution are indicated with 6.1 and 5.1 in A, A’, B’, C’, and D’. An unknown oligomer is indicated with U in B’, C’, and D’. Chromatograms of compost extract or compost extract incubated with WAX were shifted 10–30 sec to the left as compared to endoxylanase hydrolyzed WAX.