Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
Fig 2
GSI treatment reduces T effector numbers and effector differentiation in vivo.
A. 2D2 transfer model. T cells from 2D2 TCR transgenic, CD90.1 congenic mice were isolated and transferred on day -1. Mice were immunized with MOG35-55/CFA on day 0. Mice were treated with vehicle (DMSO) or GSI beginning on day -1 as indicated (arrows) and cell responses were detected on day 7 post-immunization. B. Total splenic CD4+ T cell counts. C. Percentages of donor (CD90.1+) among CD4 T cells. D. Total numbers of donor CD4 T cells present in the spleen. E. Percentage of donor T cells that had diluted CFSE. F-K Impact of GSI on Th1 differentiation in vivo. F. Histogram overlay of Tbet expression. G. Comparison of Tbet expression (MFI) in donor T cells. H. Numbers of donor cells expressing Tbet. I. the total number of IFNγ+ donor T cells in the spleen. J. Numbers of host-derived CD4+ cells expressing Tbet. K. the total number of IFNγ+ donor T cells in the spleen. L-Q GSI treatment alters Th17 responses L. Histogram overlay of RORγt expression. M. Comparison of RORγt expression (MFI) in donor T cells. N. Numbers of donor cells expressing RORγt. O. the total number of IL-17+ donor T cells in the spleen. P. number of host-derived T cells expressing RORγt. Q. Number of IL-17+ host-derived T cells. Symbols indicate results from individual mice. Open circles indicate treatment with DMSO, filled squares indicate GSI treatment. Error bars indicate standard deviation. Asterisks indicate significant differences (* p<0.05).