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BACE1-cleavage of Sez6 and Sez6L is elevated in Niemann-Pick type C disease mouse brains

Fig 9

NPC1 mouse primary cortical neurons show profound Sez6L punctuate staining in neuronal processes.

Representative images of primary mouse cortical neurons stained with Sez6L antibody and endocytic markers: early endosome antigen (EEA1) and transferrin receptor (TfR). Immunoreactivity of Sez6L in NPC1+/+ (wt) cortical neurons was detected in both soma and neuronal processes. Upon NPC1-loss and cholesterol accumulation in NPC1-/- (NPC1) cortical neurons Sez6L showed more enlarged punctate staining mainly in TfR-positive vesicles in the soma and in neuronal processes (indicated by arrows).

Fig 9

doi: https://doi.org/10.1371/journal.pone.0200344.g009