The selectivity of galardin and an azasugar-based hydroxamate compound for human matrix metalloproteases and bacterial metalloproteases
Fig 4
Structural superimposition (backbone) of the 5cuh and the 1l6j x-ray structures of MMP-9.
The 1l6j structure contains the FnII domains (yellow) and the catalytic domain (green), while the 5cuh only contains the catalytic domain (blue). The pro-domain has been deleted from 1l6j, such that the sequence starts at F107. The co-crystallized hydroxamate inhibitor LT4 of the 5cuh in red, while the catalytic zinc and the coordinating histidines are in grey. The position of the co-crystallized inhibitor was used to define the docking grid during docking of galardin and compound 1b, and the figure shows that the docking into a structure lacking the FnII domains (5cuh) should not affect the docking results.