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Surface functionalization of polyurethane scaffolds mimicking the myocardial microenvironment to support cardiac primitive cells

Fig 5

Hydrolytic and enzymatic degradation of bare and LN1-functionalized PU scaffolds: Weight loss, changes in morphology, loss of molecular weight and cytotoxicity of degradation products.

Weight loss profiles of PU and PU-LN1 scaffolds undergoing (A) hydrolytic and (B) enzymatic degradation. (C) Mn loss profiles of PU scaffolds during hydrolytic and enzymatic degradation. (D) SEM micrographs of PU-LN1 scaffolds during hydrolytic and enzymatic degradation. (E) Viability of NIH-3T3 cells in the presence of Dulbecco’s Modified Eagle’s Medium (DMEM) containing PU degradation products (0.1 mg/mL) compared to control conditions, as evaluated by Cell Titer Blue assay (n = 4).

Fig 5

doi: https://doi.org/10.1371/journal.pone.0199896.g005