Susceptibility of influenza viruses to hypothiocyanite and hypoiodite produced by lactoperoxidase in a cell-free system
Fig 6
LPO substrate preference of Influenza B virus inactivation is strain-dependent.
(A) The antiviral action of the cell-free H2O2/LPO/(SCN-/I-) system was tested against influenza B strains: B/Yamagata/16/1988, B/Great Lakes/1739/1954 and B/New York/1056/2003. Viruses were incubated in the presence or absence of the components of the cell-free system as indicated for 1 hour and viral inactivation was assessed by plate-forming unit assay using MDCK cells. Mean+/-S.E.M., n = 5. One-way ANOVA, Dunn’s multiple comparison test. (B) B/Yamagata/16/1988 Virus inactivation was measured at increasing I- concentrations (0.4–40 mM) in the cell-free system by the PFU assay. Mean+/-S.E.M., n = 5. One-way ANOVA, Dunn’s multiple comparison test. (C) The extents of IBV inactivation by OSCN- and OI- of the three strains tested were compared. Mean+/-S.E.M., Mann-Whitney test. Ns, not significant; *, p<0.05; **, p<0.01; ***, p<0.001. SCN-, thiocyanate; OSCN-, hypothiocyanite; I-, iodide; OI-, hypoiodite; LPO, lactoperoxidase; GO, glucose oxidase; MDCK, Madin-Darby canine kidney cells; PFU, plaque-forming unit; IBV, Influenza B virus.