Susceptibility of influenza viruses to hypothiocyanite and hypoiodite produced by lactoperoxidase in a cell-free system
Fig 4
Hypoiodite is more efficient in inactivating H3N2 influenza A viruses than hypothiocyanite in the cell-free system.
(A) The antiviral action of the cell-free H2O2/LPO/(SCN-/I-) system was tested against H3N2 influenza A strains: A/Texas/50/2012 (n = 3), A/Wisconsin/67/2005 (n = 2), A/Hong Kong/8/68 (n = 4) and A/Aichi/2/68 (n = 5). Viruses were incubated in the presence or absence of the components of the cell-free system as indicated for 1 hour and number of viable viruses was assessed by plate-forming unit assay using MDCK cells. Mean+/-S.E.M. One-way ANOVA, Dunn’s multiple comparison test. (B) Virus inactivations of the H3N2 strains are compared according to the LPO substrates used. Mean+/-S.E.M., n = 2–5. Mann-Whitney test. IAV, Influenza A virus; SCN-, thiocyanate; I-, iodide; LPO, lactoperoxidase; GO, glucose oxidase; MDCK, Madin-Darby canine kidney cells; PFU, plaque-forming unit.