Characterization of a non-nudix pyrophosphatase points to interplay between flavin and NAD(H) homeostasis in Saccharomyces cerevisiae
Fig 6
Growth of wild-type and mutant yeast under different conditions.
The OD600 values were measured in a microplate (path length ~0.6 cm). Yeast were grown by flask culture in YP medium (1% yeast extract, 2% peptone) supplemented with 2% glucose (A, B), 3% glycerol (C); and 1% sodium acetate (D), under either normal (A, C, D) or hypoxic (B) conditions. Data are given as the average ± S.E. of three separate cultures. Symbols are: solid circles, wild-type; open circles, Δfpy1; solid triangles, Δflx1; open triangles, Δflx1/Δfpy1.