Characterization of a non-nudix pyrophosphatase points to interplay between flavin and NAD(H) homeostasis in Saccharomyces cerevisiae
Fig 3
Potassium requirement for the FAD pyrophosphatase activity of Fpy1p.
(A) Enzyme activity in the absence or presence of 200 mM KCl and/or 200 mM NaCl when assayed with 4 mM CoCl2. (B) Enzyme activity in the absence or presence of 200 mM KCl and/or 200 mM NaCl when assayed with 8 mM MgCl2. (C) Enzyme activity as a function of KCl concentration in the presence of 4 mM CoCl2 (solid circles) or 10 mM MgCl2 (open circles). Data are given as the average ± S.E. of three triplicate determinations.