Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish
Fig 3
Imaging neutrophils in larval zebrafish.
(A) A maximum intensity projection of a three-dimensional light sheet fluorescence image of GFP-expressing neutrophils near the intestine of a 5 dpf larval zebrafish. The 3D scan is provided as S2 Movie. The intestine and swim bladder are roughly outlined by the yellow dotted lines. Scale bar: 100 μm. (B) The total number of neutrophils in each fish; the x-axis is ordered by neutrophil count. (C) Neutrophil count along the anterior-posterior dimension, summed over all fish examined (N = 41). The x-axis corresponds approximately to the horizontal range of (A). (D) The total intensity of the detected neutrohils per fish vs the total number of neutrophils in that fish. The two measures are weakly correlated with a coefficient of determination R2 = 0.4.