Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish
Fig 2
Specimen positioning and image quality.
(A) Composite brightfield image of a larval zebrafish positioned in a glass capillary. Scale bar: 500 μm. (B) Normalized intensity averaged along the short axis of the brightfield image, and the intensity of the template image that best matches the fish in (A). Cross-correlation with the template is used to automatically position the fish for light sheet fluorescence imaging. (C) Light sheet fluorescence images of a 28 nm diameter fluorescent microsphere, showing x-y and z-y planes centered on the particle. (D) Line-scan of intensity along the detection axis (z) through a fluorescent microsphere, with a Gaussian fit showing a width of approximately 3 μm.