Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish
Fig 1
(A) Schematic of the instrument design, with labels corresponding to the parts list in S1 Table. See also S1 Movie. The excitation laser line is selected by an acousto-optic tunable filter (AOTF2), then directed to a galvanometer mirror (G3) and objective lens (L4) to create a time-averaged sheet of light in the sample chamber (C) via a prism (Pr5). Specimens flow through a system of tubing controlled by a syringe pump (Pu9) and valves (V10) and are automatically positioned in a square-walled capillary (Cap11) for imaging. Bright field images are used for positioning the sample and are illuminated with an LED (LED6). After imaging, specimens are directed into a reservoir (R). (B) Schematic of the imaging area. The 3D-printed sample chamber (C), prism (Pr5), and imaging capillary (Cap11) are apparent. (C) Photograph of the imaging area corresponding to the schematic in (B).