Interplay of a non-conjugative integrative element and a conjugative plasmid in the spread of antibiotic resistance via suicidal plasmid transfer from an aquaculture Vibrio isolate
Fig 5
Tn6283 excision barely generates unoccupied donor sites in vivo.
(A) A model for Tn6283 excision in E. coli strain LN5. Three models are proposed (see main text for details). Primer sets used for qPCR are indicated as arrows. (B). A model for Tn6283 excision in V. ponticus. (C) Copy numbers of recombination joints in E. coli. Copy numbers of two recombination joints (attTn6283, attB*) shown as the ratio of the number of joints to the number of replicon backbone (dxs) or total Tn6283 molecule (intA). In one sample, the copy number of attB* was below the detection limit. (D) Copy numbers of recombination joints in V. ponticus. Copy numbers of two recombination joints (attTn6283, Scar1) shown as the ratio of the number of joints to the number of pSEA1 backbone (traI) or total Tn6283 molecules (intA). Each dot indicates total DNA extracted from one batch of stationary-phase cultures. The amount of Scar1 was too low to detect in a quantitative manner (outside the range of standard curve), and is thus shown as the numbers below the detection limit (red line: 10−7).