Modification of N6-methyladenosine RNA methylation on heat shock protein expression
Fig 5
Effect of YTHDF2 on HSPs mRNA expression and cell viability in HepG2 cells.
YTHDF2 knockdown decreased YTHDF2 mRNA in HepG2 cells (A). Expression of HSPA1B (HSP70), HSPA9 (HSP70), HSPB1 (HSP27), HSP90AA1 (HSP90), HSPD1 (HSP60) mRNA from the sample of YTHDF2 knockdown in HepG2 cells (B and C). The relative cell viability determined by MTT at 24, 48, and 72 h after knockdown of YTHDF2 with or without heat shock pretreatment (D) (n = 6). Data are shown as mean ± SEM. *p value ≤ 0.05, **p value ≤ 0.01.