Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard
Fig 6
Statistical power comparison between AS and AUC.
Statistical power is plotted against the PMG differences between the two comparing groups. Sigmoidal curves are simulated with different PMG (-16.0959, -.0959 and -24.0959 for the three panel columns). The variance for each measurement of the simulated curve is either constant or changing along the curve with a linear or quadratic relationship with the measurement values on the curve in the three panel rows. Statistical power is calculated based on the two group t test. AUC is estimated based on quadratic curve fitting and integration. Var, variance.