Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard
Fig 4
Relationship between the three proxies and simulated PMG.
Sigmoidal curves are simulated with different point of maximum growth (PMG). Each estimated proxy is plotted against the simulatied PMG. The estimated PMG works well for values where there is more data surrounding the PMG, but variance increases when the available data are not near the PMG. The endpoint titer method results in discrete values and has a weaker relationship with the actual PMG. The absorbance summation has a strong relationship with the true PMG.