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Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard

Fig 2

Examples of successful and unsuccessful sigmoidal curve fitting.

A. Successful sigmoidal curve fit where the data cover the estimated point of maximum growth (PMG). B. Successful sigmoidal curve fit even though data are not available for the estimated PMG. C. Unsuccessful sigmoidal curve fit due to the lack of informative data for fitting the curve. Line, fitted sigmoidal curve.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0198528.g002