Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
Fig 4
Cardenolides are potent inhibitors of RBM20 dependent titin splicing.
Figure layout and panels as described in Fig 3. (a, b) Additional cardenolides not present in the original library were selected to evaluate their potency to inhibit RBM20 dependent titin splicing. The structural analogs digoxin and digitoxin both efficiently inhibit RBM20 mediated splicing. (DLR N = 4; RNA N = 3). ***P<0.001 versus CTRL (Dunnett’s post-test). Data are presented as mean ±SD.