Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula
Fig 1
Nod-LCO induced plasma membrane potential depolarizations in root hairs of two M. truncatula genotypes.
(A) Transmitted light image of a M. tuncatula root hair impaled with a microelectrode. Scale bar represents 10 μm. (B) Representative membrane potential changes evoked by application of 100 nM Nod-LCO (NodSM-IV, C16:2) to the bath solution. Membrane potential changes are shown of root hair cells of the genotypes A17 (closed circle) and R108-1 (open circle). (C) Average changes in plasma membrane potential of M. truncatula root hairs, stimulated with 100 nM Nod-LCOs (NodSM-IV, C16:2, S, triangles and open circles), or control solution (open rectangles). The time point of Nod-LCO application is indicated by the arrow. Data are shown for root hair cells of M. truncatula genotype A17 (n = 11, open circles) and R108-1 (n = 37, closed triangles). Error bars indicate SE.