Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production
Fig 7
The timeline for development of stable MGAT1- CHO cell lines expressing HIV-1 rgp120.
Leading clones expressing 0.2–0.4 g/L in shake flasks under standard laboratory conditions were selected in less than two months. Production was subsequently increased to levels of g/L rgp120 production with minimal feed optimization.