Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production
Fig 6
Binding of bN-mAbs to A244-rgp120 produced in normal and A244_N332-rgp120 produced in MGAT1- CHO cell lines.
A244_N332-rgp120 was purified from the stable clone 5F MGAT1- CHO cell line (closed circles) or from the MGAT1- CHO cells (open circles) transiently transfected with the UCSC 1331 plasmid. A244-rgp120 expressed and purified from transiently transfected CHO-S cells (open squares). Antibody binding was measured by a fluorescent immunoassay (FIA).