Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production
Fig 2
Primary identification of high producer MGAT1- CHO lines expressing A244_N332 rgp120 by immunofluorescent labeling.
(A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the ClonePix2 plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.