Thiol-maleimide poly(ethylene glycol) crosslinking of L-asparaginase subunits at recombinant cysteine residues introduced by mutagenesis
Fig 5
Reusability of the 1kDa-PEG-conjugate.
Relative catalytic activity at temperatures ranging from 20°C to 80°C. The 1kDa-PEG-conjugate (in green), native L-asparaginase (in blue), and a commercial randomly-PEGylated L-asparaginase formulation (Millipore Sigma, USA) (in black). Relative catalytic activity was calculated by the absorption at 425 nm divided by the maximum signal for each sample and expressed as percentile (%). Reported values are average with error bars representing the 95% confidence interval. For the 1kDa-PEG-conjugate the same sample (semi-solid gel) was used throughout all the experiment.