Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages
Fig 5
S1pr2 deletion suppresses the expression of profibrotic genes in BALF cells.
The mRNA expression levels of M1 and M2 markers and related genes (A-N) in BALF cells from wild-type (WT) and S1pr2-/- mice on days 7, 22 and 33 after starting administration of bleomycin or saline were determined by qPCR. The open and solid bars indicate the values of saline–and bleomycin (Bleo)–administered mice, respectively. The individual values and the mean ± SEM are shown. Each sample was prepared from the pooled cells obtained from 3–4 mice. N = 3−6 from three independent experiments. *P <0.05, **P <0.01 and ***P<0.001 (two-way ANOVA followed by the Bonferroni post-test).