Constitutive metanephric mesenchyme-specific expression of interferon-gamma causes renal dysplasia by regulating Sall1 expression
Fig 5
The expression of Sall1 and its potential targets are modulated in mutant kidneys.
E14.5 kidneys were used for all analyses. A. semi-quantitative RT-PCR for Sall1. The expression level of Sall1 mRNA is decreased in mutants. B. Immunostaining of Sall1. Sall1 protein expression is also decreased in mutants. Lower: higher magnification of areas shown in the white rectangles. C. Active β-Catenin is determined by immunoblotting with a β-Catenin antibody that detects unphosphorylated residues at Ser33/Ser37/Thr41, which target the molecule for ubiquitination. β-Catenin is activated in mutants. D. RT-PCR of Wnt9b and Axin2. The mRNA levels of Wnt9b and Axin2, a target gene of Wnt/β-Catenin signaling, are increased in mutants. E. semi-quantitative RT-PCR for Kif26b. The mRNA level of Kif26b, a target gene for Sall1, is reduced in mutants. nor—normal, mu—mutant.