Cell electrofusion based on nanosecond/microsecond pulsed electric fields
Fig 1
Schematic diagram of cell fusion using a sequential nanosecond/microsecond electric field pulse combination.
100-ns-long strong field pulse induced many tiny pores in the cell membrane, particularly in the junction region. After a brief delay, fusion process was followed by a low-field 10-microsecond pulse, which enlarged the pores.