Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens
Fig 5
Exosomal RNAs are stable over two decades.
Dot plots of total RNA yield and OD260/OD280 from 105 EQ-treated archival patient samples using the RNeasy Mini Kit combined with TRIzol LS. Storage time for each sample is indicated on the x-axis. The Spearman’s rank correlations for RNA yield versus storing time and OD260/OD280 versus storage time are 0.185 (P = 0.06) and 0.04 (P = 0.70), respectively.