Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens
Fig 3
Comparison of sera exosomal RNA using four different RNA extraction methods.
(A) Total RNA yield from ultracentrifugation (UC) and ExoQuick (EQ) treated samples using the RNeasy Mini Kit combined with TRIzol LS, the RNeasy Mini Kit alone, conventional RNA precipitation, and AllPrep DNA/RNA Mini Kit. (B) Demonstration of RNA quality measured by OD260/OD280 in EQ and UC treated samples. Data are shown as the mean ± SD from six independent patient samples. ***P<0.001, **P<0.01, *P<0.05, ns, not significant, student’s t test. Note: RNA could not be extracted from some samples using the AllPrep kit due to clogging.