Isolation and molecular characterization of novel glucarpidases: Enzymes to improve the antibody directed enzyme pro-drug therapy for cancer treatment
Fig 8
Carboxypeptidase G2 activity is Zn2+ dependent.
MTX substrate solution and pure CPG2 in the presence of Zn2+ (star), control reaction of MTX substrate solution and buffer (filled triangle) and MTX substrate solution and pure CPG2 in the presence of Zn2+ and 10 mM EDTA (square) are shown. New isolated CPG2 is Zn2+ dependent.