Isolation and molecular characterization of novel glucarpidases: Enzymes to improve the antibody directed enzyme pro-drug therapy for cancer treatment
Fig 3
Glucarpidase activity in the total soluble protein of the two novel glucarpidase producing bacteria.
MTX substrate solution and total soluble protein of Pseudomonas lubricants strain SF168 in presence of Zn2+ (filled ball) and in presence of Zn2+ and EDTA (diamond). MTX substrate solution and total soluble protein from Xenophilus sp. SN213 in the presence of Zn2+ (star), and in the presence of Zn2+ and EDTA (filled triangle), and control of MTX and enzyme buffer (square) are shown. The data in this figure indicates that both strains (Pseudomonas lubricans strain SF168 and Xenophilus sp. SN213) show CPG2 activity through methotrexate hydrolysis.