Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers
Fig 4
Responses of different proteins in the Bradford assay.
Different proteins were assayed by the Bradford method using the cuvette format (1 ml of assay) under control conditions (panel A) or after denaturation in 10 μl of SDS buffer and in the presence of 2.5 mg alpha-cyclodextrin/ ml of Bradford reagent (panel B). Black: bovine serum albumin. Purple: hen conalbumin. Blue: soybean trypsin inhibitor. Green: hen lysozyme.