Scaffold hopping from (5-hydroxymethyl) isophthalates to multisubstituted pyrimidines diminishes binding affinity to the C1 domain of protein kinase C
Fig 7
Representative binding curves for HMI 1a3, pyrimidines 1e, 1g, 2d, 2l and PMA.
Binding of [3H]PDBu (10 nM) to purified PKCα measured in the presence of increasing concentrations of the tested compounds. The data is presented as mean of residual [3H]PDBu binding (% of control) from three parallel samples in a single representative experiment. The raw data of the displacement assays are available in S2 File.