MicroRNA expression in serum samples of sulfur mustard veterans as a diagnostic gateway to improve care
Fig 2
Comparative differential microRNA analysis workflow including sampling controls.
The panel (A) shows that two different analysis approaches were applied. On the left side the IPC and delta Ct method followed by linear model / Bayes based differential analysis was performed while on the right side a pure linear model / Bayes approach was used based on the results of the pilot analysis. The relevance of both approaches was tested by a resampling approach. The right branch was finally chosen and basis for the discussion. In panel (B) the results of all the alternative tests on either the normal-mild or the normal-severe comparisons are shown. The results on the right side indicate that in the normal-mild comparison 15 and in the normal-severe comparison 29 microRNAs are stable on a 5% significance level after applying the resampling control. The numbers on white background indicate the remaining candidates after the intersections were performed. The result denotes a good consistency between left and right procedure.