A high-throughput 3’ UTR reporter screening identifies microRNA interactomes of cancer genes
Fig 5
3’ UTR reporter rescue experiment.
Rescue of 3’ UTR reporter regulation. (A) For four MYCN interactions significant down-regulation of reporter activity after miRNA expression modulation can no longer be demonstrated upon canonical binding site mutation (one-sided t-test; p < 0.001 ***; p < 0.01 **; wt = wild-type 3’ UTR; mut = mutant 3’ UTR) in two independently replicated reporter experiments. Reporter activity is expressed relative to non-targeting miRNA treated controls (NTC). Error bars represent standard deviations on three technical replicates. Successful rescue of MYCN regulation could only be achieved in one experiment for hsa-miR-494. (B) For four hsa-miR-449 interactions significant down-regulation of reporter activity after miRNA expression modulation can no longer be demonstrated upon canonical binding site mutation (one-sided t-test; p < 0.001 ***; p < 0.01 **; wt = wild-type 3’ UTR; mut = mutant 3’ UTR) in two independently replicated reporter experiments. Reporter activity is expressed relative to non-targeting miRNA treated controls (NTC). Error bars represent standard deviations on three technical replicates. Successful rescue of regulation by hsa-miR-449 could only be achieved in one experiment for MYB.