Histone acetyltransferase TGF-1 regulates Trichoderma atroviride secondary metabolism and mycoparasitism
Fig 10
TGF-1 and TSA differentially regulate T. atroviride ech-42, prb-1, pbs-1 and tps-1 in the presence or absence of R. solani.
Dual culture assays of T. atroviride wt and Δtgf-1 against R. solani, or grown alone as control were performed on PDA in absence (A, B, C, D) or in presence of 300 nM TSA (E, F, G, H) at 28 °C. Total RNA was extracted from T. atroviride mycelium collected at 36, 48, and 60 h. Relative expression was calibrated using act-1 as housekeeping gene and normalized against the wt strain in absence of the phytopathogen. ech-42 = 42-kDa endochitinase gen, prb-1 = basic proteinase gene, pbs-1 = peptaibol synthase gene, tps-1 = terpene synthase gene. Black bars represent Δtgf-1 strain, whereas gray bars represent Δtgf-1 vs R. solani interaction. Addition of TSA is indicated at the top of the panels. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly (P < 0.05). This assay was repeated twice per triplicate with similar results.