Histone acetyltransferase TGF-1 regulates Trichoderma atroviride secondary metabolism and mycoparasitism
Fig 3
TSA and R. solani presence negatively affected T. atroviride ech-42, prb-1, pbs-1, and tps-1 gene expression.
T. atroviride and R. solani were co-cultured on PDA (A, B, C, and D) or PDA amended with 300 nM TSA (E, F, G, and H) at 28 °C. Total RNA was extracted from T. atroviride mycelium collected at 36, 48, and 60 h. Relative expression was calibrated using act-1 as housekeeping gene and normalized against the wt strain in the absence of the phytopathogen. ech-42 = 42-kDa endochitinase gene, prb-1 = basic proteinase gene, pbs-1 = peptaibol synthase gene, and tps-1 = terpene synthase gene. Black bars, T. atroviride wt strain growing alone (Ta); grey bars, T. atroviride vs R. solani (Ta+Rs) co-cultures. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly (P < 0.05). This assay was repeated twice per triplicate with similar results.