Mycophenolic acid induces senescence of vascular precursor cells
Fig 6
Guanosine reversed MPA’s inhibitory effect on cell proliferation and senescence markers.
Cell proliferation using trypan blue exclusion assay shows that guanosine is not cytostatic or inhibitory (A), and that addition of guanosine rescues ECFC proliferation in a dose dependent manner (B). Relative fold expression of senescence associated gene markers p16INK4a, p21WAF and p27KiP1(C). Phosphorylation of p53 normalized to total p53 level in cell lysates (D). Results represent the mean ± SD compared (n = 3). *P < 0.05, ***P <0.001 relative to vehicle. Abbreviation: MPA = mycophenolic acid, ECFC = endothelial colony forming cell, OD = optical density, ph-p53 = phosphorylated p53, tot-p53 = total p53.