Mycophenolic acid induces senescence of vascular precursor cells
Fig 4
Diminished ECFC vasculogenic function after MPA treatment.
Left panel shows representative photomicrographs (magnification, 5x) of TdTomato ECFC following no treatment (vehicle) or treatment with 2.5 μM MPA in 2D assay (A) and 3D assay (D). 2D Matrigel assay showed that there is decreased average total cord length (C) and cord area (B) after MPA treatment. 3D collagen assay showed decreased total vessel area (E) after MPA treatment. *P <0.05, **P <0.005 compared to vehicle (n = 3). Abbreviations: ECFC = endothelial colony forming cells, 2D = two-dimensional, 3D = three-dimensional, EGM = endothelial growth medium, MPA = mycophenolic acid, mm = millimeter.