In silico and in vitro studies of the reduction of unsaturated α,β bonds of trans-2-hexenedioic acid and 6-amino-trans-2-hexenoic acid – Important steps towards biobased production of adipic acid
Fig 2
Metabolic pathways for the conversion of lysine to adipic acid.
Our alternative pathway. Different types of enzymatic reactions are shown in different colors: removal of NH2 from α-carbon (yellow), reduction of unsaturated α,β bond (pink), removal of the terminal NH2 (green), and oxidation of aldehyde to carboxylic acid (blue). The enzymatic reactions targeted in this study are encircled. The reaction numbers according to the Kyoto Encyclopedia of Genes and Genomes are given for known reactions. The E.C. numbers according to the Braunschweig Enzyme Database are given for known enzymes. akg = alpha-ketoglutaric acid, Glu-L = L- glutamic acid.