Targeted liquid chromatography tandem mass spectrometry to quantitate wheat gluten using well-defined reference proteins
Fig 2
Precursor to product ion transition (m/z) of each marker peptide (P1-16) and the isotopically labelled standard (*P11).
Marker peptides were quantitated in the respective protein type of wheat (multiple reaction monitoring mode, MRM). Two MRM transitions were monitored for each peptide and the most abundant MRM transition shown here was used for quantitation. HMW-GS, high-molecular-weight glutenin subunits; LMW-GS, low-molecular-weight glutenin subunits.