Targeted liquid chromatography tandem mass spectrometry to quantitate wheat gluten using well-defined reference proteins
Fig 1
Schematic diagram showing the development of a method for the quantitation of gluten contents based on peptide yields.
(A) Peptide identification and selection of 16 wheat marker peptides, (B) development of the liquid chromatography tandem mass spectrometry (LC-MS/MS) method with an isotopically labelled peptide as internal standard and optimization of the LC-MS/MS conditions, (C) quantitation of peptide yields in reference gluten protein types and conversion of peptide into protein type and gluten concentrations.