Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841
Fig 8
Solvent accessible channels in BiuH.
Three solvent accessible channels emerging from the active site are shown in blue, green, and yellow. A) The blue and green tunnels quickly reach the surface of the monomer, while the yellow tunnel extends into the subunit interface and central cavity of the tetramer. The amino acids constituting the active site are shown as sticks, and the subunit that contained biuret is shown in pale green. B) The active site residues are shown as green sticks (for a representative structure of cluster 0) and as grey lines (for representative structures of other 19 clusters) and biuret is shown as Van der Waals spheres. The residues that are responsible for gating these tunnels are labeled in red C) The three dominant tunnels are shown on the right (green, yellow, and blue). Figure generated with Caver [52].