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Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841

Fig 1

Cyanuric acid mineralization by bacteria.

Cyanuric acid is mineralized to CO₂ and NH₃ by cyanuric acid mineralizing bacteria by the enzymes cyanuric acid amidohydrolase, biuret hydrolase and allophanate hydrolase. The product of cyanuric acid amiidohydrolase (1-carboxybiuret) is unstable under physiological conditions and decarboxylates to form biuret and CO₂. The product of allophanate amidohydrolase (dicarboxyammonia) is also unstable under physiological conditions and decomposes to CO₂ and NH₃.

doi: https://doi.org/10.1371/journal.pone.0192736.g001