Establishment of an Arabidopsis callus system to study the interrelations of biosynthesis, degradation and accumulation of carotenoids
Fig 5
β-Apocarotenoids in Arabidopsis callus.
A, β-Apocarotenals were determined in seed-derived calli from AtPSY-overexpressing (At12, At22) and ZmPSY1-expressing (Zm18) Arabidopsis lines by LC-MS analyses. Apocarotenoid amounts were expressed in ng g-1 DM. β-IAc, β-ionylidene acetaldehyd. The amounts of β-carotene (in μg g-1 DM) are given for comparison. B, β-ionone, C, β-cyclocitral and D, 5,6-epoxy-β-ionone are expressed as peak areas normalized to internal standards and dry mass. Results are means ± SD from three biological replicates. Significant difference to the WT, *P<0.05.